Device

Part:BBa_M36805:Design

Designed by: Richard Li   Group: Stanford BIOE44 - S11   (2014-05-03)


Acetaldehyde Sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 872
    Illegal XbaI site found at 942
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 872
    Illegal XbaI site found at 942
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 872
    Illegal XbaI site found at 942
    Illegal AgeI site found at 1623
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1407


Design Notes

Codons were chosen to minimize >10bp repeats.


Source

AlcR ORF was derived from Teleromyces stipitatus, and codon-optimized for E. coli.

References